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Workflow for intestinal organoid generation and monolayer preparation (A) Schematic representation of tissue processing, crypt isolation, and embedding in BME domes to establish 3D intestinal organoids. (B) Overview of organoid dissociation and seeding onto wells to generate organoid-derived monolayers (ODMs). (C) BME domes containing organoids; (D) <t>Bright</t> <t>field</t> image of organoids ready for ODM seeding; (E) Bright field image of organoids not suitable for ODM seeding. They have overgrown and there are too many dead cells in the lumen. Scale bar: 100 μm. Created with Biorender.com.
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Workflow for intestinal organoid generation and monolayer preparation (A) Schematic representation of tissue processing, crypt isolation, and embedding in BME domes to establish 3D intestinal organoids. (B) Overview of organoid dissociation and seeding onto wells to generate organoid-derived monolayers (ODMs). (C) BME domes containing organoids; (D) <t>Bright</t> <t>field</t> image of organoids ready for ODM seeding; (E) Bright field image of organoids not suitable for ODM seeding. They have overgrown and there are too many dead cells in the lumen. Scale bar: 100 μm. Created with Biorender.com.
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Workflow for intestinal organoid generation and monolayer preparation (A) Schematic representation of tissue processing, crypt isolation, and embedding in BME domes to establish 3D intestinal organoids. (B) Overview of organoid dissociation and seeding onto wells to generate organoid-derived monolayers (ODMs). (C) BME domes containing organoids; (D) Bright field image of organoids ready for ODM seeding; (E) Bright field image of organoids not suitable for ODM seeding. They have overgrown and there are too many dead cells in the lumen. Scale bar: 100 μm. Created with Biorender.com.

Journal: STAR Protocols

Article Title: Protocol to enhance pre-sexual and sexual differentiation of Toxoplasma gondii using retinal cells and intestinal organoid-derived monolayers

doi: 10.1016/j.xpro.2026.104367

Figure Lengend Snippet: Workflow for intestinal organoid generation and monolayer preparation (A) Schematic representation of tissue processing, crypt isolation, and embedding in BME domes to establish 3D intestinal organoids. (B) Overview of organoid dissociation and seeding onto wells to generate organoid-derived monolayers (ODMs). (C) BME domes containing organoids; (D) Bright field image of organoids ready for ODM seeding; (E) Bright field image of organoids not suitable for ODM seeding. They have overgrown and there are too many dead cells in the lumen. Scale bar: 100 μm. Created with Biorender.com.

Article Snippet: Bright-field inverted microscope (20× 0.3 NA and 40× 0.8 NA objectives) , Zeiss , Primovert.

Techniques: Isolation, Derivative Assay